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Improvement of pectinase production by interspecific hybrids of Aspergillus strains
Author(s) -
Solís S.,
Flores M. E.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00352.x
Subject(s) - library science , interspecific hybrids , microbiology and biotechnology , biology , hybrid , botany , computer science
Protoplast fusion induced by polyethylene glycol and Ca 2+ , was performed between auxotrophic mutants of pectinolytic fungi Aspergillus sp. CH‐Y‐1043 (A13) ade − and Aspergillus flavipes ATCC‐16795 (F7) lys − . Prototrophic colonies were developed on minimal medium with a fusion frequency of 1·0×10 −2 . The reversion frequency of the mutation in spores and protoplasts was low and ranged from 2·0 to 4·0×10 −7 . Four prototrophic hybrids (HH, HE, HF and HJ) exhibited enhanced production of endo‐pectinase and pectin‐lyase. The highest production was observed in HJ ; maximum activities were 150 and 160% respectively, whereas the exo‐pectinase production was similar to the wild‐type strain Aspergillus sp. CH‐Y‐1043. Hybrid HJ showed the greatest growth ; nevertheless, specific endo‐pectinase and pectin‐lyase activities were higher in all hybrids than those produced by the wild‐type strains.