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PCR detection of sequences similar to the AS‐48 structural gene in bacteriocin‐producing enterococci
Author(s) -
Joosten H. M. L. J.,
Rodriguez E.,
Nuñez M.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00349.x
Subject(s) - bacteriocin , biology , enterococcus faecium , microbiology and biotechnology , enterococcus faecalis , gene , nested polymerase chain reaction , enterococcus , polymerase chain reaction , oligonucleotide , antimicrobial , peptide , genetics , antibiotics , escherichia coli , biochemistry
Three oligonucleotide primers, specific for the structural gene of antimicrobial peptide AS‐48, were used to study the distribution of sequences similar to this gene in 15 independently isolated bacteriocin‐producing Enterococcus strains by semi‐nested PCR. Eight of 10 Ent. faecalis strains and three of five Ent. faecium strains gave a positive result in both reactions of the semi‐nested PCR. These findings indicate that bacteriocins produced by many enterococcal strains are very closely related or even identical to peptide AS‐48.