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Isolation and characterization of a novel catalase‐negative, urease‐positive Campylobacter from cattle faeces
Author(s) -
Atabay H. I.,
Corry J. E. L.,
On S. L. W.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00347.x
Subject(s) - cefoperazone , microbiology and biotechnology , biology , campylobacter , agar , pulsed field gel electrophoresis , feces , urease , enrofloxacin , nalidixic acid , agar plate , gentamicin , tetracycline , bacteria , antibiotics , ciprofloxacin , antibiotic resistance , imipenem , enzyme , biochemistry , genotype , genetics , gene
Forty‐four strains of a phenotypically unique Campylobacter were isolated from the faeces of 26 of 45 cows in a single herd. Isolation involved enrichment and membrane filtration onto blood agar or plating onto cefoperazone amphotericin teicoplanin agar. The strains exhibited phenotypic characteristics typical for Campylobacter species. However, they were unusual in that they produced urease and copious H 2 S in triple sugar iron (TSI) medium, but did not produce catalase. They did not grow aerobically. None of the strains grew on modified cefoperazone charcoal deoxycholate agar (mCCDA). Macrorestriction profiles of chromosomal DNA were prepared for 15 strains using pulsed‐field gel electrophoresis (PFGE). Twelve of 15 profiles were identical and all appeared to be closely related. These catalase‐negative, urease‐positive campylobacters (CNUPC) represent a group not previously reported. Their sensitivity to antibiotics normally used in selective media for campylobacters might explain why they have not previously been encountered. Their ecological significance and importance with respect to human and animal disease remain to be assessed.