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Glucosyltransferase activity of commercial juice‐processing enzyme preparations
Author(s) -
Hang Y. D.,
Woodams E. E.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00344.x
Subject(s) - maltose , chemistry , glucosyltransferase , enzyme , chromatography , sodium acetate , alpha glucosidase , biochemistry , food science
Of various commercial enzyme preparations examined, Cytolase M102 was found to contain the highest glucosyltransferase activity (55 U ml −1 ). It rapidly converted maltose to panose (Glcα1 → 6Glcα1 → 4Glc) with a V max value of 5·8 mmol l −1 min −1 at 50°C in 0·05 mol l −1 sodium acetate buffer (pH 4·4). The K m value of the enzyme for maltose was 750 mmol l −1 . Yields of panose and glucose after 45 min of reaction, for example, were 47·2% and 52·8%, respectively, on the basis of the amount of maltose consumed.