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Quantitative determination of the antifungal compound 6‐pentyl‐α‐pyrone (6PAP) using a simple plate bioassay
Author(s) -
Cooney J. M.,
Vanneste J. L.,
Lauren D. R.,
Hill R. A.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00342.x
Subject(s) - bioassay , spore , agar , agar plate , pyrone , chromatography , antifungal , cladosporium , biology , strain (injury) , microbiology and biotechnology , detection limit , chemistry , bacteria , stereochemistry , aspergillus , genetics , anatomy
An assay relating bioactivity to levels of the antifungal compound 6‐pentyl‐α‐pyrone (6PAP) has been developed. Using Cladosporium strain NZ660US as indicator at a spore concentration of 5×10 6 spores overlay −1 on malt (adjusted to pH 3·5) agar plates, this assay allows quantitative determination of 6PAP down to a detection limit of 0·5% v/v. This will allow rapid screening of Trichoderma culture extracts to compare the bioactivity of isolates grown under different culture conditions, and can be used in combination with analytical chemical analysis to identify the presence of other bioactive metabolites.