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Gene amplification (PCR) to detect and differentiate mycoplasmas in porcine mycoplasmal pneumonia
Author(s) -
Stemke G.W.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00243.x
Subject(s) - mycoplasma hyopneumoniae , biology , polymerase chain reaction , pneumonia , mycoplasmataceae , mollicutes , serology , virology , microbiology and biotechnology , mycoplasma , nested polymerase chain reaction , mycoplasma pneumoniae , atypical pneumonia , gene , dna , genome , respiratory disease , antibody , mycoplasma pneumonia , lung , immunology , genetics , medicine
The causative agent of porcine mycoplasmal pneumonia, Mycoplasma hyopneumoniae, is difficult and time‐consuming to isolate. Serological identification using antibodies induced by the disease is confused by cross‐reaction with a closely related organism, Myc. flocculare . From pig lungs obtained at slaughter for meat and deemed free of acute disease, it was possible to detect by culture both Myc. hyopneumoniae and Myc. flocculare . This study has improved on an earlier PCR detection of DNA from the former species by using a nested PCR capable of detecting the purified DNA equivalent to one mycoplasmal genome. With this PCR assay both mycoplasma species were detected and differentiated directly from lung tissue.