Premium
An immuno‐dot blot assay for detection of thermostable protease from Pseudomonas sp. AFT‐36 of dairy origin
Author(s) -
Matta H.,
Punj V.,
Kanwar S.S.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00228.x
Subject(s) - proteases , protease , pseudomonas , detection limit , dot blot , microbiology and biotechnology , biology , chromatography , chemistry , enzyme , bacteria , biochemistry , dna , genetics
A dot‐ELISA technique for the detection of Pseudomonas protease was developedusing IgG of anti‐ Pseudomonas AFT‐36 protease as capture antibody. The detection limitof protease in buffer or milk was 1·01 ng ml −1 . The procedure was performedat room temperature, took about 2·5 h and was economical. Protease AFT‐36 isimmunologically related to five out of seven Pseudomonas spp. The results suggest thatthe assay could be used to detect proteases in dairy products.