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Chitinolytic activity of an endophytic strain of Bacillus cereus
Author(s) -
Pleban S.,
Chernin L.,
Chet I.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00224.x
Subject(s) - bacillus cereus , chitinase , rhizoctonia solani , isoelectric point , cereus , microbiology and biotechnology , chitin , biology , enzyme , strain (injury) , enzyme assay , chemistry , biochemistry , bacteria , botany , genetics , anatomy , chitosan
Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis , wasshown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. Theenzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose(GlcNAc) 2 from the non‐reducing end of trimeric chitin derivatives. The chitinaseexhibited activity over the pH range 4·5–7 5 and was stable between pH 4·0 and8·5. The enzyme had an isoelectric point of 6·4.Application of B. cereus 65directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctonia solani .