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Restriction fragment length polymorphism analysis shows that the hippuricase gene of Campylobacter jejuni is highly conserved
Author(s) -
Slater E.R.,
Owen R.J.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00218.x
Subject(s) - campylobacter jejuni , campylobacter , public health , biology , restriction fragment length polymorphism , microbiology and biotechnology , genetics , gene , library science , medicine , genotype , bacteria , nursing , computer science
A 1151‐bp amplicon containing the hippuricase ( hip O) gene was obtained from 118strains of Campylobacter jejuni and double‐digested with Alu I and Dde I togive five different PCR‐RFLP patterns. Most strains had the six‐banded profile predicted fromsequence data. Lack of polymorphisms within the hip O gene indicated it was highlyconserved amongst strains of Camp. jejuni , and that RFLP analysis provided only lowdiscrimination as an epidemiological typing method. Detection of hip O by PCR provided auseful test for confirmatory identification of Camp. jejuni .