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Use of antibody‐coated cellulose sponges for enhanced isolation of salmonella
Author(s) -
Davies R.H.,
Wray C.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00211.x
Subject(s) - salmonella , cellulose , antiserum , sponge , biology , isolation (microbiology) , microbiology and biotechnology , bacteria , food science , antibody , biochemistry , botany , genetics , immunology
One thousand, four hundred and fifty‐one naturally contaminated samples from pig, poultry andcattle farms, poultry hatcheries and animal feed mills were examined in a trial in which transfer ofsmall portions of cellulose sponge coated with salmonella somatic polyvalent antiserum wascompared with transfer of standardliquid inocula from pre‐enrichment to selective enrichmentculture. Salmonella wasfound in 281 (19·4%) of the samples using the standard method,compared with385 (26·5%) using the sponge method. It was therefore concluded thatantibody‐coatedcellulose sponges could be a simple means of increasing the recovery ofsalmonellasfrom pre‐enrichment broths and thereby enhancing the test sensitivity.