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Subspecies differentiation of Salmonella by PCR‐RFLP of the ribosomal operon using universal primers
Author(s) -
Shah S. A.,
Romick T. L.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00174.x
Subject(s) - subspecies , biology , restriction fragment length polymorphism , operon , ribosomal rna , polymerase chain reaction , salmonella , serotype , genetics , bacteria , identification (biology) , 16s ribosomal rna , microbiology and biotechnology , gene , escherichia coli , paleontology , botany
A polymerase chain reaction (PCR)‐based method was developed to aid identification of bacteria to subspecies level. The method used primers that annealed to highly conserved regions of the bacterial rRNA operon, which are proposed to be universal for all bacteria. The resulting PCR products gave unique electrophoretic patterns due to restriction fragment length polymorphisms (RFLP) within the rRNA operon, allowing differentiation to the subspecies level. Six serotypes of Salmonella choleraesuis are presented to demonstrate the specificity of PCR‐RFLP patterns for building an identification database. As the database continues to accumulate, the method proves to be specific and rapid for identifying bacteria based on stable genetic characteristics.