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Production and detection of muramidase and acetylglucosaminidase from Agaricus bisporus
Author(s) -
Lincoln S. P.,
Fermor R.,
Wood D. A.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00163.x
Subject(s) - agaricus bisporus , microbiology and biotechnology , muramidase , biology , agaricus , chemistry , food science , lysozyme , biochemistry , mushroom
The production and regulation of extracellular bacteriolytic enzymes of Agaricus bisporus are being studied to understand better the nutrition of this fungus and to identify factors that regulate the selectivity of mushroom compost as a growth medium. Both muramidase (EC.3.2.1.17) and N ‐acetyl‐β‐ D ‐glucosaminidase (β‐GlcNAcase, EC.3.2.1.30) have been detected in liquid cultures of A. bisporus , and in cultures fruiting in sterile and non‐sterile compost. A turbidometric assay, based on the decrease in optical density of suspended Bacillus subtilis bacterial cell walls, was used to measure muramidase production by A. bisporus . A colorimetric assay was used to measure β‐GlcNAcase. Both bacteriolytic enzyme activities were produced on a range of sole carbon sources, including killed freeze‐dried B. subtilis cells. Muramidase activity was highest in axenic compost cultures. Bacteriolytic enzyme activity peaked as the first group of fruit bodies was harvested in both sterile and non‐sterile compost.