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Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water
Author(s) -
Iqbal S.,
Robinson J.,
Deere D.,
Saunders J.R.,
Edwards C.,
Porter J.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00160.x
Subject(s) - polymerase chain reaction , escherichia coli , hot start pcr , recombinase polymerase amplification , multiple displacement amplification , biology , microbiology and biotechnology , dna , gene , gene duplication , polymerase chain reaction optimization , inverse polymerase chain reaction , multiplex polymerase chain reaction , genetics , dna extraction
Direct detection of Escherichia coli from polluted river water was achieved using polymerase chain reaction (PCR) amplification of the uid gene. Amplification using DNA from environmental samples resulted in non‐specific DNA fragments. Specific amplification was achieved through use of the touch‐down PCR procedure. Targeting the uidA structural region of the gene gave reproducibly better amplification than targeting the uidR regulatory region. The data demonstrate conditions for optimal specific detection.