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β‐Xylosidase and xylanase characterization and production by Streptomyces sp. CH‐M‐1035
Author(s) -
Flores M. E.,
Pérez R.,
Huitrón C.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00149.x
Subject(s) - xylanase , xylan , bagasse , streptomyces , food science , extracellular , pith , sugar , chemistry , biochemistry , streptomycetaceae , biology , enzyme , actinomycetales , microbiology and biotechnology , botany , bacteria , genetics
Extracellular xylanase activity and cell‐bound β‐xylosidase production by a selected strain of Streptomyces sp. CH‐M‐1035 was characterized during growth on three xylans, sugar cane bagasse pith and lemon peel as sole carbon source. The cell‐bound β‐xylosidase and extracellular endoxylanase had pH optima of 6·0 and 5·0, and temperature optima of 50°C and 60°C, respectively. The highest level of β‐xylosidase activity was obtained when Streptomyces sp. CH‐M‐1035 was grown on larchwood xylan, whereas the maximal endoxylanase production was found on lemon peel. Reducing sugars accumulated in the culture media when Streptomyces sp. CH‐M‐1035 was grown on xylans, but not on agroindustrial residues.