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Detection of Pseudomonas putida B in the rhizosphere by RAPD
Author(s) -
Hansen B.M.,
Winding A.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00126.x
Subject(s) - rhizosphere , microbial ecology , pseudomonas putida , library science , rapd , ecology , biology , microbiology and biotechnology , sociology , bacteria , genetics , computer science , population , demography , genetic diversity
A method was developed for the detection of Pseudomonas putida B MM12 released into the rhizosphere of non‐sterile barley, using a Random Amplified Polymorphic DNA (RAPD)‐generated probe for hybridization with RAPD products generated from DNA extracted from the rhizosphere. The detection procedure involves extraction of rhizosphere bacteria by sonication, extraction of DNA by boiling, RAPD and Southern hybridization with RAPD products and the selected probe. The level of detection of MM12 was at least 1·9×10 4 cells g −1 barley root. MM12 was detected in rhizosphere when it constituted as little as 0·5% of the culturable population.