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VNTR derived oligonucleotides as PCR primers for population studies in filamentous fungi
Author(s) -
Bridge P.D.,
Pearce D.A.,
Rivera A.,
Rutherford M.A.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00060.x
Subject(s) - biology , polymerase chain reaction , population , genetics , microbiology and biotechnology , strain (injury) , gene , demography , anatomy , sociology
One M13 phage derived and three simple sequence repeat primers were assessed for the development of a standard set of PCR primers for population studies with filamentous fungi. Fungal isolates from five plant or insect pathogenic genera with ascomycete and basidiomycete affinities were screened. Three of the four primers were found to be suitable for generating ‘genetic fingerprints’ for all of the fungal genera tested. Strain groupings obtained from the individual primers were found to be supportive through constrained two‐dimensional cluster analysis.