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Rapid purification of extracellular tannase using polyethylene glycol–tannic acid complex
Author(s) -
Gupta R.,
Bradoo S.,
Saxena R. K.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00054.x
Subject(s) - tannic acid , tannase , polyethylene glycol , chemistry , extracellular , chromatography , precipitation , protein precipitation , peg ratio , enzyme , biochemistry , high performance liquid chromatography , organic chemistry , gallic acid , physics , finance , meteorology , economics , antioxidant
This paper reports on a rapid procedure for partial purification of extracellular tannase using a combination of tannic acid and PEG‐6000. A pH‐dependent protein precipitation was obtained within 30 min. Monitoring the primary precipitation curve of the culture filtrate at varying pH, a second step was designed that yielded an approximately eight‐fold increase in the enzyme purification. This procedure is of importance as no temperature and pH monitoring is required and a very fast precipitation is obtained without any alteration in the enzymatic properties of the precipitant.