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Interaction of potassium channel openers with glioma cells mitochondria
Author(s) -
Dębska G.,
Kicińska A.,
Skalska J.,
Szewczyk A.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.22_9.x
Subject(s) - bk channel , biophysics , chemistry , conductance , depolarization , membrane potential , potassium channel , mitochondrion , membrane , inner mitochondrial membrane , biochemistry , biology , physics , condensed matter physics
Benzimidazolones such as NS1619 and NS004 have been reported to activate plasma membrane large conductance Ca 2+ activated potassium channel (BK‐type K (Ca) ). In the present study we have examined the effect of NS1619 and NS004 plasma membrane BK‐ channel openers on mitochondria in glioma cells. We described the effect of BK‐channel openers on mitochondrial membrane potential (ΔΨ m ), on mitochondrial respiration and membrane bilayer conductance. We have shown that BK channel openers caused significant decrease in ΔΨ m with an EC 50 value of 3.6 ± 0.4 μ m for NS1619 and 5.4 ± 0.8 μ m for NS004. This membrane depolarization was produced by inhibition of mitochondrial respiratory chain, probably complex I. Both BK‐channel openers had no effect on membrane bilayer current or conductance. Acknowledgement: This work was supported by State Committee for Scientific Research grant 6P04A01219.