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Effect of sphingosine‐1‐phosphate on calcium signaling in cerebellar astrocytes and neurons
Author(s) -
Viani P.,
Ferraretto A.,
Gravaghi C.,
Giussani P.,
Bassi R.,
Tettamanti G.,
Riboni L.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.22_2.x
Subject(s) - second messenger system , microbiology and biotechnology , sphingosine , extracellular , activator (genetics) , signal transduction , sphingosine 1 phosphate , biology , receptor , intracellular , calcium signaling , inositol phosphate , cell signaling , inositol , biochemistry
Sphingosine‐1‐phosphate (SP1P) is a sphingoid molecule implicated in the control of many biological processes in different cells including those of the nervous system. Also in brain cells SP1P can function as an extracellular signal, through binding to specific receptors, or as second messenger of stimuli able to activate sphingosine kinase. SP1P has been reported to directly act on internal Ca 2+ stores or induce a Ca 2+ signal through the coupling of its receptors with PLC. We investigated the effect of SP1P on Ca 2+ signaling in cells of the nervous system and found that administration of SP1P induced an increase of [Ca 2+ ]i in astrocytes but not in differentiated granule cells. SP1P stimulated the production of inositol phosphates and increased [Ca 2+ ]i by mobilization from intracellular stores, thus acting as an extracellular mediator in astrocytes. Our data suggest SP1P may act as activator of calcium‐mediated events and play a role in glial function and dysfunction.