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The influence of Id1 protein on human stem cells differentiation into neuronal pathway
Author(s) -
Jurga M.,
Bużańska L.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.21_4.x
Subject(s) - neurogenesis , neural stem cell , biology , progenitor cell , electroporation , stem cell , microbiology and biotechnology , cellular differentiation , transfection , cell culture , gene , genetics
Id1 protein is responsible for inhibition of bHLH transcription factors. It lacks basic region necessary for DNA binding and after dimerization with other bHLH proteins, competitively blocks their function. Relatively high expression of Id1 in cancer cells suggests its role in promoting cell division. To study the influence of Id1 protein on human neural stem/progenitor cells differentiation Dev and HUCB‐NS/P (human umbilical cord blood derived neural stem/progenitor) cell lines were applied. Id1 gene in sense and antisense orientation was introduced into cells with pEGFP‐N1 vector by electroporation. Overexpression of Id1 results in de‐differentiation of the cells in both experimental models. In contrast, transfection with antisense Id1 results in promotion of neuronal differentiation but only in HUCB‐NS/P cells. These results show the influence of Id1 on neural stem cells differentiation and its unique selectivity referred to neurogenesis inhibition. Acknowledgements: Funded by Polish State Committee for Scientific Research (grant no. 76P05A.04920).