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Ferritin in Parkinsonian and control brains
Author(s) -
Koziorowski D.,
Friedman A.,
GałązkaFriedman J.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.19_8.x
Subject(s) - ferritin , substantia nigra , globus pallidus , parkinson's disease , oxidative stress , chemistry , endocrinology , medicine , biology , biochemistry , basal ganglia , disease , central nervous system
Ferritin, the main iron storage protein, may be involved in triggering oxidative stress injury in Parkinson's disease. Aim: To determine the ratio of H and L chains of ferritin and the size of its iron‐core in brain areas with high amounts of iron: substantia nigra (SN), globus pallidus (GP) and hippocampus (Hip). Methods: H/L ratio was determined by ELISA and iron size of ferritin by direct measurements of isolated ferritin with the use of electron microscopy (EM). Material: ELISA was made on 11 control and four Parkinsonian GP, Hip and SN. EM data were obtained from measurements of 50 iron‐cores of ferritin in control GP, SN and Hip. Results: H/L ratios in control brains vs. PD brains were 5.92 ± 1.65 vs. 9.45 ± 1.93 for GP, 5.05 ± 1.67 vs. 11.41 ± 3.47 for SN, 16.01 ± 9.86 vs. 24.85 ± 5.88 for Hip. The sizes of iron‐cores in control brains were 3.3 ± 0.5 nm for GP, 3.7 ± 0.5 nm for SN and 3.1 ± 0.5 nm for Hip. These results may suggest different iron metabolism both in different brain areas and between PD and control.