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Biotransformation of D‐panthenol and its action in protection of activation of oxidizing processes in brain
Author(s) -
Moiseenok A. G.,
Kanunnikova N. P.,
Gurinovich V. A.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.19_10.x
Subject(s) - chemistry , biotransformation , biochemistry , in vivo , malondialdehyde , bioavailability , oxidizing agent , pharmacology , oxidative stress , enzyme , organic chemistry , biology , microbiology and biotechnology
It is a well known fact that 3H‐panthenol (PL) has a high bioavailability, so we studied its biotransformation and its protective action against lipoperoxide activation in homogenates and mitochondrial‐synaptosomal fraction (11 000 g) of rat brain. The lipoperoxidation was initialized by Fe 2+ ‐ascorbate complex (Fe 2+ ‐Asc). In experiments in vivo , after 30 min, we demonstrated accumulation of intermediate products of CoA biosynthesis – pantothenic acid (PA), phospho‐PA, and phosphopantetheine – in postmitochondrial fraction of brain, by using a HPLC technique. Addition of the PL (10 m m ) to brain hemispheres homogenates or mitochondrial‐synaptosomal fraction caused a remarkable reduction of malondialdehyde production. However, 30 min preincubation with the PL, but not with PA, was ineffective. The data obtained may be a reason for a high neuroprotective activity of PL in curing brain diseases with vessel or alcohol‐induced damages.