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The study of the creatine kinase in rat brain during ischemia by magnetization transfer and biochemical analysis
Author(s) -
Dobrota D.,
Likavčanová K.,
Kašparová S.,
Liptaj T.,
Prónayová N.,
Mlynárik V.,
Belan V.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.17_2.x
Subject(s) - creatine kinase , magnetization transfer , ischemia , creatine , chemistry , in vivo , nuclear magnetic resonance , brain ischemia , endocrinology , magnetic resonance imaging , medicine , biochemistry , biology , physics , microbiology and biotechnology , radiology
Various methods are used to study the biochemical changes in the central nervous system under normal and pathological conditions. The magnetization transfer 31 P magnetic resonance technique was used here to measure the creatine kinase (CK) reaction rate constant in vivo in rats with cerebral ischemia. The measurements indicated that the rate constant of the CK reaction was significantly reduced in the case of chronic brain ischemia in aged rats. The similar reduction of the creatine kinase activity was found in the ischemic rat brain homogenate measured by biochemical analysis. At the same time, corresponding conventional phosphorus magnetic resonance spectra showed negligible or no change in signal intensities of compounds containing macroergic phosphates. Acknowledgements: This work was supported by the Grant Category C and Comenius University Grant No. X/2003.