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Changes of expression of mitochondrial proteins involved in energy transduction in rat brain during aging
Author(s) -
Nicoletti V. G.,
Marino V. M.,
Giuffrida Stella A. M.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.16_8.x
Subject(s) - adenine nucleotide translocator , protein subunit , biology , cerebellum , mitochondrion , cerebral cortex , respiratory chain , cortex (anatomy) , mitochondrial respiratory chain , oxidative phosphorylation , microbiology and biotechnology , biochemistry , neuroscience , gene
Oxidative damage is the major responsible for mitochondrial dysfunction during aging and many neurodegenerative diseases with respiratory chain Complex I mostly affected. A down‐regulation of the mitochondrial respiratory chain Complex‐I subunits ND1 (mitochondrial encoded) in rat total cerebral cortex RNA and 39 kDa (nuclear encoded) in frontal cortex protein extracts was observed at 6, 12 and 28 months of age. However the 39 kDa subunit showed an age‐related up‐regulation in the parietal cortex and no changes in the cerebellum protein extracts. In frontal and parietal cortex protein extracts a significant reduction of subunit α (nuclear encoded) of F o F 1 ‐ATP synthase, with no changes in the cerebellum, were also observed. A similar impairment was found also for mitochondrial adenine nucleotide translocator ANT‐1 in total rat cerebral cortex RNA. Any change of subunit synthesis might lead to neuronal dysfunction and increase of ROS.