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Proteomics in neurodegenerative diseases
Author(s) -
Sagi D.,
Zabel C.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.85.s2.16_4.x
Subject(s) - proteomics , ageing , disease , biology , proteome , protein aggregation , microbiology and biotechnology , computational biology , bioinformatics , biochemistry , genetics , medicine , pathology , gene
Many neurodegenerative diseases share a common feature: aggregation and deposition of abnormal proteins. The cellular mechanisms that cause these diseases can be studied by animal models. A novel approach to determine changes in protein expression is proteomics, i.e. separation of complex protein mixtures by two‐dimensional‐electrophoresis and subsequent identification of the proteins by mass spectrometry. In this contribution Huntington's and Parkinson's disease have been studied in two distinct mouse model systems using this approach. We identified disease‐related proteins further upstream or downstream of the main disease pathway and proteins changed as a consequence of disease. Moreover, we found up‐/down‐regulated proteins which both diseases have in common. By comparing these results with protein changes associated with ageing in mice we identified shared protein changes as well. These data indicate that neurodegenerative diseases and ageing possibly affect the same cellular pathways.