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Effects of ethanol on gangliosides in the plasma, liver, and brain of inbred mouse strains
Author(s) -
Saito M. I.,
Saito M. A.,
Szakall I.,
Vadasz C.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.81.s1.3_18.x
Subject(s) - ganglioside , inbred strain , ethanol , immunostaining , c57bl/6 , chemistry , biology , ratón , biochemistry , microbiology and biotechnology , endocrinology , medicine , immunology , gene , immunohistochemistry
The mouse inbred strains C57BL/6ByJ and BALB/cJ show genetically different alcohol‐related behaviors. Using these strains, we examined ganglioside contents in the plasma, liver, and brain with or without acute ethanol treatment. The quantification of GM1 was performed with a TLC‐immunostaining procedure using choleragenoid, and the contents of other gangliosides were measured after staining with resorcinol reagents. It is known that there are polymorphisms in ganglioside compositions among inbred mouse strains. We found that the plasma GM1 level in BALB/cJ mice (1.6 ± 0.6 ng/μL) was 12 times higher than the level found in C57BL/6ByJ mice (0.13 ± 0.03 ng/μL) although the major ganglioside in both strains was GM2. The ganglioside profiles in the liver were similar to those of the plasma, and the GM1 level in BALB/cJ was 25 times higher than that of C57BL/6ByJ. The liver probably synthesizes the plasma gangliosides as suggested in other studies. The total brain ganglioside compositions were also different between BALB/cJ and C57BL/6ByJ. The levels of GD1b and GQ1b were higher in BALB/cJ although the GM1 contents were similar. These animals were injected with 20% ethanol intraperitoneally in a single dose of 3 g/kg and the ganglioside contents were measured after 4 h. The GM1 levels in the liver and plasma were lower in ethanol‐treated animals while the GM1 levels were higher in erythrocytes and brains. Since it has been shown that the administration of GM1 attenuates or modifies the effects of ethanol in several animal models, the difference in GM1 contents in the plasma between BALB/cJ and C57BL/6ByJ may contribute to some of the differences in ethanol‐related behaviors or toxicity between these strains.