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P2X 7 receptor induces gene expression
Author(s) -
SantosBerríos C.,
Weisman G. A.,
Gonzalez F. A.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.81.s1.1_3.x
Subject(s) - extracellular , biology , receptor , gene expression , tumor necrosis factor alpha , microglia , microbiology and biotechnology , nucleotide , monocyte , gene , biochemistry , immunology , inflammation
The concentration of extracellular nucleotides is known to rise significantly under inflammatory conditions in vivo . In normal tissues, extracellular nucleotides are maintained at low concentrations and tightly regulated. However, activated lymphocytes, macrophages, microglia, and platelets, as well as cells undergoing necrosis or apoptosis, release high concentrations of different nucleotide diphosphates and triphosphates into the extracellular space. It has also been shown that P2 nucleotide receptors ligands modulate IL‐1β‐induced transcription factor activation and differentially regulate inflammatory gene expression in primary human astrocytes. In previous work using cDNA microarray technology and RT–PCR, we found that chronic exposure of human monocytic U937 cells to UTP modulated the expression of a large number of genes related to the monocyte to macrophage differentiation process, including the tumor necrosis factor (TNF‐α). Here we present the effect of chronic exposure to extracellular nucleotides and nucleotide analogs on the expression of genes in 1321 N1 astrocytoma cells expressing recombinant P2X 7 receptors. Our findings will help us better understand the role of nucleotide receptors in the inflammatory response in the nervous system, information that is bound to have pharmacological applications and clinical significance.