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From hematopoiesis to neuropoiesis: evidence of overlapping genetic programs
Author(s) -
Terskikh A. V.,
Easterday M. C.,
Li L.,
Hood L.,
Kornblum H. I.,
Geschwind D. H.,
Weissman I. L.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.81.s1.105.x
Subject(s) - biology , stem cell , haematopoiesis , neurosphere , neural stem cell , progenitor cell , adult stem cell , microbiology and biotechnology , neurogenesis , population , gene , cellular differentiation , genetics , demography , sociology
Hematopoietic stem cells (HSC) are clonogenic cells that can self‐renew and give rise to both lymphoid and hematopoietic systems throughout the life span. Using cDNA subtraction, we have identified a set of genes selectively expressed in mouse adult HSC as opposed to its direct microenvironment, whole bone marrow. Some of these genes were found to be expressed in the subventricular zone of fetal and adult brains, one of the regions of continious neurogenesis. Using cDNA microarray techniques we confirmed gene expression in mouse neurospheres, a population enriched for neural stem cells, and down‐regulation in differentiated cells. We propose that transcripts enriched in several types of stem cells define a functionally conserved group of genes likely to include the regulatory genes involved in self‐renewal of stem cells. To address the self‐renewal potential of stem cell associated genes we have used retroviral transfer into highly purified HSC and primitive neural progenitor cells.