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Homodimerization of adenosine A 2A receptors: qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer
Author(s) -
Canals Meritxell,
Burgueño Javier,
Marcellino Daniel,
Cabello Núria,
Canela Enric I.,
Mallol Josefa,
Agnati Luigi,
Ferré Sergi,
Bouvier Michel,
Fuxe Kjell,
Ciruela Francisco,
Lluis Carmen,
Franco Rafael
Publication year - 2004
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2003.02200.x
Subject(s) - förster resonance energy transfer , receptor , biophysics , biotinylation , hek 293 cells , chemistry , agonist , g protein coupled receptor , transfection , cell surface receptor , cell , biochemistry , fluorescence , biology , gene , physics , quantum mechanics
The results presented in this paper show that adenosine A 2A receptor (A 2A R) form homodimers and that homodimers but not monomers are the functional species at the cell surface. Fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) techniques have been used to demonstrate in transfected HEK293 cells homodimerization of A 2A R, which are heptaspanning membrane receptors with enriched expression in striatum. The existence of homodimers at the cell surface was demonstrated by time‐resolved FRET. Although agonist activation of the receptor leads to the formation of receptor clusters, it did not affect the degree of A 2A R–A 2A R dimerization. Both monomers and dimers were detected by immunoblotting in cell extracts. However, cell surface biotinylation of proteins has made evident that more than 90% of the cell surface receptor is in its dimeric form. Thus, it seems that homodimers are the functional form of the receptor present on the plasma membrane. A deletion mutant version of the A 2A receptor, lacking its C‐terminal domain, was also able to form both monomeric and dimeric species when cell extracts from transfected cells were analyzed by immunoblotting. This suggests that the C‐terminal tail does not participate in the dimerization. This is relevant as the C‐terminal tail of A 2A R is involved in heteromers formed by A 2A R and dopamine D2 receptors. BRET ratios corresponding to A 2A R–A 2A R homodimers were higher than those encountered for heterodimers formed by A 2A R and dopamine D2 receptors. As A 2A R and dopamine D2 receptors do indeed interact, these results indicate that A 2A R homodimers are the functional species at the cell surface and that they coexist with A 2A R/D2 receptor heterodimers.