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RNA editing and alternative splicing of human serotonin 2C receptor in schizophrenia
Author(s) -
Dracheva Stella,
Elhakem Sharif L.,
Marcus Sue M.,
Siever Larry J.,
McGurk Susan R.,
Haroutunian Vahram
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2003.02115.x
Subject(s) - rna editing , alternative splicing , rna splicing , gene isoform , serotonin , messenger rna , biology , receptor , rna , 5 ht receptor , schizophrenia (object oriented programming) , microbiology and biotechnology , genetics , gene , psychology , psychiatry
Serotonin 2C receptor (5‐HT 2C R) heterogeneity in the brain occurs mostly from two different sources: (i) 5‐HT 2C R mRNA undergoes adenosine‐to‐inosine editing events at five positions, which leads to amino acid substitutions that produce receptor variants with different pharmacological properties; (ii) 5‐HT 2C R mRNA is alternatively spliced, resulting in a truncated mRNA isoform (5‐HT 2C R‐tr) which encodes a non‐functional serotonin receptor. 5‐HT 2C R mRNA editing efficiencies and the expression of the full‐length and the truncated 5‐HT 2C R mRNA splice isoforms were analyzed in the prefrontal cortex of elderly subjects with schizophrenia vs. matched controls (ns = 15). No significant differences were found, indicating that there are no alterations in editing or alternative splicing of 5‐HT 2C Rs that are associated with schizophrenia in persons treated with antipsychotic medications. Quantitation of 5‐HT 2C R and 5‐HT 2C R‐tr mRNA variants revealed that the expression of 5‐HT 2C R‐tr was ∼ 50% of that observed for the full‐length isoform.