Premium
Uncoupling protein 2 influences dopamine secretion in PC12h cells
Author(s) -
Yamada Shigeru,
Isojima Yasushi,
Yamatodani Atsushi,
Nagai Katsuya
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2003.02005.x
Subject(s) - intracellular , secretion , protein kinase a , dopamine , microbiology and biotechnology , biology , stimulation , excitatory postsynaptic potential , chemistry , inhibitory postsynaptic potential , endocrinology , medicine , phosphorylation
Uncoupling protein 2 (UCP2) belongs to the UCP family, and is distributed in many organs including the brain. Although UCP2 is known to be related to many functions such as the regulation of insulin secretion or the scavenging of the radicals, the role of UCP2 in the central nervous system remains unclear. In this report, rat UCP2 (rUCP2) and its mutants were overexpressed in the PC12h cells to determine the physiological roles played by UCP2 in neural cells and to elucidate the mechanisms that regulate these functions. It was found that rUCP2 was activated by the stimulation of the cAMP‐protein kinase A (PKA) cascade. Moreover, the activation of rUCP2 suppressed intracellular ATP levels and inhibited the cAMP‐dependent increase of dopamine secretion. Thus, UCP2 appears to be regulated by the excitatory stimulus via the cAMP‐PKA cascade and serves to negatively control the synaptic output by reducing intracellular ATP levels.