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Ero1‐L , an ischemia‐inducible gene from rat brain with homology to global ischemia‐induced gene 11 ( Giig11 ), is localized to neuronal dendrites by a dispersed identifier (ID) element‐dependent mechanism
Author(s) -
Chen Dexi,
Jin Kunlin,
Kawaguchi Kenji,
Nakayama Masaki,
Zhou Xiaoman,
Xiong Zhigang,
Zhou An,
Mao Xiao Ou,
Greenberg David A.,
Graham Steven H.,
Simon Roger P.
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2003.01699.x
Subject(s) - fusion protein , in situ hybridization , biology , suppression subtractive hybridization , ischemia , microbiology and biotechnology , gene , messenger rna , complementary dna , gene expression , endoplasmic reticulum , hippocampal formation , cdna library , neuroscience , recombinant dna , medicine , genetics
Many changes in neuronal gene expression occur in response to ischemia, and these may play a role in determining the fate of ischemic neurons. To identify genes induced in the rat brain following cerebral ischemia, a strategy was used that combines subtractive hybridization and differential screening. Among the genes identified was one referred to as global ischemia‐inducible gene 11( Giig11 ). Sequence analysis indicated that Giig11 exhibited 97% and 91% identity to the known Ero1‐L ( S. cereviseae ero1‐ like oxidoreductase) of mouse and human origin, which is involved in oxidative endoplasmic reticulum protein folding. Rat Ero1‐L / Giig11 also contains a l07‐bp sequence that is nearly identical (> 95%) to the known dispersed repetitive identifier (ID), but which is lacking in mouse and human Ero1‐L . Northern blotting showed that expression of the ID element and Ero1‐L / Giig11 mRNA increased after global cerebral ischemia. In situ hybridization demonstrated increased expression of Ero1‐L / Giig11 in the brain following ischemic injury, with the highest levels in the vulnerable hippocampal CA1 pyramidal neurons. Transfection of cultured primary hippocampal neurons with a plasmid containing green fluorescent protein ( gfp ) and Ero1‐L / Giig11 cDNA (with and without the ID element) produced a gfp‐Ero1‐L / Giig11 fusion protein, and more fusion protein was localized into dendrites in the presence of the ID element, suggesting that the ID element promotes Ero1‐L / Giig11 protein localization to dendrites. Therefore, Ero‐1L / Giig11 may have a role in ischemia‐induced neuronal repair or survival mechanisms directed at counteracting abnormalities in protein folding, maturation and distribution.