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Differences in multidrug resistance phenotype and matrix metalloproteinases activity between endothelial cells from normal brain and glioma
Author(s) -
Régina Anthony,
Demeule Michel,
Bérubé Alexandra,
Moumdjian Robert,
Berthelet France,
Béliveau Richard
Publication year - 2003
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2003.01521.x
Subject(s) - phenotype , glioma , matrix metalloproteinase , mmp9 , biology , efflux , blood–brain barrier , endothelium , cancer research , brain tumor , microbiology and biotechnology , pathology , gene , neuroscience , central nervous system , medicine , endocrinology , genetics , downregulation and upregulation
Endothelial cells (ECs) are new targets for tumor therapy. In this work, we purified endothelial cells from intracerebral and subcutaneous experimental gliomas as well as from normal brain in order to define some of the phenotypical differences between angiogenic and quiescent brain vasculature. We show that the multidrug resistance genes encoding drug efflux pumps at the brain endothelium are expressed differently in normal and tumoral vasculature. We also show that ECs from gliomas present increased activity of gelatinase B (MMP9), key enzyme in the angiogenic process. Importantly, we observe a different phenotype between ECs in the intracerebral and subcutaneous models. Our results provide molecular evidence of phenotypic distinction between tumoral and normal brain vasculature and indicate that the EC phenotype depends on interactions both with tumor cells and also with the microenvironment.