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Type‐specific inositol 1,4,5‐trisphosphate receptor localization in the vomeronasal organ and its interaction with a transient receptor potential channel, TRPC2
Author(s) -
Brann Jessica H.,
Dennis John C.,
Morrison Edward E.,
Fadool Debra A.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2002.01266.x
Subject(s) - vomeronasal organ , biology , olfactory epithelium , transient receptor potential channel , receptor , microbiology and biotechnology , inositol trisphosphate receptor , sensory system , sensory receptor , olfaction , inositol , neuroscience , biochemistry
The vomeronasal organ (VNO) is the receptor portion of the accessory olfactory system and transduces chemical cues that identify social hierarchy, reproductive status, conspecifics and prey. Signal transduction in VNO neurons is apparently accomplished via an inositol 1,4,5‐trisphosphate (IP 3 )‐activated calcium conductance that includes a different set of G proteins than those identified in vertebrate olfactory sensory neurons. We used immunohistochemical (IHC) and SDS–PAGE/western analysis to localize three IP 3 receptors (IP 3 R) in the rat VNO epithelium. Type‐I IP 3 R expression was weak or absent. Antisera for type‐II and ‐III IP 3 R recognized appropriate molecular weight proteins by SDS–PAGE, and labeled protein could be abolished by pre‐adsorption of the respective antibody with antigenic peptide. In tissue sections, type‐II IP 3 R immunoreactivity was present in the supporting cell zone but not in the sensory cell zone. Type‐III IP 3 R immunoreactivity was present throughout the sensory zone and overlapped that of transient receptor potential channel 2 (TRPC2) in the microvillar layer of sensory epithelium. Co‐immunoprecipitation of type‐III IP 3 R and TRPC2 from VNO lysates confirmed the overlapping immunoreactivity patterns. The protein–protein interaction complex between type‐III IP 3 R and TRPC2 could initiate calcium signaling leading to electrical signal production in VNO neurons.