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SEL‐10 interacts with presenilin 1, facilitates its ubiquitination, and alters A‐beta peptide production
Author(s) -
Li Jinhe,
Pauley Adele M.,
Myers Rick L.,
Shuang Rongqing,
Brashler John R.,
Yan Riqiang,
Buhl Allen E.,
Ruble Cara,
Gurney Mark E.
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2002.01105.x
Subject(s) - presenilin , ubiquitin ligase , amyloid precursor protein , biology , ubiquitin , alpha secretase , amyloid beta , microbiology and biotechnology , amyloid (mycology) , amyloid precursor protein secretase , gene , caenorhabditis elegans , peptide , alzheimer's disease , genetics , biochemistry , disease , medicine , botany
Abstract Mutations in the human presenilin genes ( PS1 or PS2 ) have been linked to autosomal dominant, early onset Alzheimer's disease (AD). Presenilins, probably as an essential part of gamma‐secretase, modulate gamma‐cleavage of the amyloid protein precursor (APP) to the amyloid β‐peptide (Aβ). Mutations in sel‐12 , a Caenorhabditis elegans presenilin homologue, cause a defect in egg laying that can be suppressed by loss of function mutations in a second gene, SEL‐10 . SEL‐10 protein is a homologue of yeast Cdc4, a member of the SCF ( S kp1‐ C dc53/CUL1‐ F ‐box protein) E2‐E3 ubiquitin ligase family. In this study, we show that human SEL‐10 interacts with PS1 and enhances PS1 ubiquitination, thus altering cellular levels of unprocessed PS1 and its N‐ and C‐terminal fragments. Co‐transfection of sel‐10 and APP cDNAs in HEK293 cells leads to an alteration in the metabolism of APP and to an increase in the production of amyloid β‐peptide, the principal component of amyloid plaque in Alzheimer's disease.