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Analysis of genes induced in peripheral nerve after axotomy using cDNA microarrays
Author(s) -
Kubo Tateki,
Yamashita Toshihide,
Yamaguchi Atsushi,
Hosokawa Ko,
Tohyama Masaya
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2002.01060.x
Subject(s) - axotomy , biology , cdna library , gene , complementary dna , sciatic nerve , regeneration (biology) , peripheral nervous system , schwann cell , microarray , microbiology and biotechnology , genetics , gene expression , anatomy , neuroscience , central nervous system
One of the most striking features of neurons in the mature peripheral nervous system is their ability to survive and to regenerate their axons following axonal injury. To perform a comprehensive survey of the molecular mechanisms that underlie peripheral nerve regeneration, we analyzed a cDNA library derived from the distal stumps of post‐injured sciatic nerve which was enriched in non‐myelinating Schwann cells using cDNA microarrays. The number of up‐ and down‐regulated genes in the transected sciatic nerve was 370 and 157, respectively, of the 9596 spotted genes. In the up‐regulated group, the number of known genes was 216 and the number of expressed sequence tag (EST) sequences was 154. In the down‐regulated group, the number of known genes was 103 and that of EST sequences was 54. We obtained several genes that were previously reported to be involved in regeneration of the injured neurons, such as cathepsin D, ninjurin 1, tenascin C, and co‐receptor for glial cell line‐derived neurotrophic factor family of trophic factors. In addition to unknown genes, there seemed to be a lot of annotated genes whose role in nerve regeneration remains unknown.