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BDNF‐mediated signal transduction is modulated by GSK3β and mood stabilizing agents
Author(s) -
Mai Lian,
Jope Richard S.,
Li Xiaohua
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2002.00939.x
Subject(s) - creb , phosphorylation , brain derived neurotrophic factor , gsk 3 , gsk3b , signal transduction , protein kinase b , medicine , endocrinology , neurotrophic factors , chemistry , microbiology and biotechnology , biology , transcription factor , biochemistry , receptor , gene
Brain‐derived neurotrophic factor (BDNF) is a major neurotrophin in the brain and abnormal regulation of BDNF may contribute to the pathophysiology of mood disorders. In the present study, we examined if alterations in the activity of glycogen synthase kinase‐3‐beta (GSK3β) or treatment with mood stabilizers modulated BDNF‐mediated signal transduction pathways in differentiated human neuroblastoma SH‐SY5Y cells. BDNF increased the phosphorylation of the forkhead transcription factor FKHRL1 through activation of the phosphatidylinositol 3‐kinase (PI3K)/Akt signaling pathway, and the phosphorylation of the cyclic AMP response element binding protein (CREB) through activation of extracellular signal‐regulated kinase1/2 (ERK1/2). BDNF also increased serine 9 ‐phosphorylation of GSK3β, which inhibits GSK3β activity. Overexpression of GSK3β did not affect BDNF‐induced phosphorylation of Akt, ERK1/2, or FKHRL1, but abolished CREB phosphorylation induced by BDNF. This inhibition of BDNF‐induced CREB phosphorylation in GSK3β‐overexpressing SH‐SY5Y cells was blocked by treatment with lithium. In contrast to lithium, sodium valproate and lamotrigine did not affect BDNF‐mediated signaling, whereas carbamazepine induced a rapid and prolonged phosphorylation of ERK1/2 and CREB in the absence or the presence of BDNF. Therefore, increased GSK3β selectively attenuates BDNF‐induced CREB phosphorylation, and lithium and carbamazepine can facilitate activation of CREB.