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Functional and molecular characterization of metabotropic glutamate receptors expressed in rat striatal cholinergic interneurones
Author(s) -
Bell Matt I.,
Richardson Peter J.,
Lee Kevin
Publication year - 2002
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2002.00815.x
Subject(s) - metabotropic glutamate receptor , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 1 , cholinergic , agonist , metabotropic receptor , neuroscience , glutamate receptor , metabotropic glutamate receptor 6 , metabotropic glutamate receptor 7 , excitatory postsynaptic potential , postsynaptic potential , biology , metabotropic glutamate receptor 2 , chemistry , pharmacology , receptor , inhibitory postsynaptic potential , biochemistry
In the present study we have used single‐cell RT‐PCR in conjunction with electrophysiology to examine the expression and functional properties of metabotropic glutamate receptors (mGluRs) expressed within biochemically identified cholinergic interneurones in the rat striatum. Using single‐cell RT‐PCR, it was possible to demonstrate the presence of mGluR1, mGluR2, mGluR3, mGluR5 and mGluR7 mRNAs within single cholinergic interneurones. Bath application of the non‐selective mGluR agonist (1 S ,3 R )‐1‐aminocyclopentane‐1,3‐dicarboxylic acid (1 S ,3 R ‐ACPD) or the group‐I mGluR agonist 3,5‐dihydroxyphenylglycine (DHPG) depolarized all cholinergic neurones tested by activation of an inward current at − 60 mV. The effects of DHPG were partially inhibited by the mGluR5 selective antagonist 6‐methyl‐2‐(pherazo)‐3‐pyridinol and by the non‐selective group‐I antagonist α‐methyl‐4‐carboxyphenylglycine but were not mimicked by the group‐II and group‐III selective mGluR agonists 2‐(2,3‐dicarboxycyclopropyl)glycine (DCG‐IV) and l ‐2‐amino‐4‐phosphonobutanoate ( l ‐AP4), respectively. Intrastriatal stimulation evoked an excitatory postsynaptic current within cholinergic neurones that was reversibly inhibited by bath application of the group‐II and group‐III selective mGluR agonists DCG‐IV and l ‐AP4, respectively, via presynaptic actions. In summary, we have identified the mGluRs expressed by striatal cholinergic interneurones and demonstrated that their activation produces modulatory effects via both pre‐ and postsynaptic mechanisms.