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Angiotensin II promotes the phosphorylation of cyclic AMP‐responsive element binding protein (CREB) at Ser133 through an ERK1/2‐dependent mechanism
Author(s) -
Cammarota Martín,
Bevilaqua Lia R. M.,
Dunkley Peter R.,
Rostas John A. P.
Publication year - 2001
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2001.00666.x
Subject(s) - creb , angiotensin ii , protein kinase a , kinase , phosphorylation , mapk/erk pathway , chemistry , cyclic amp response element binding protein , biology , microbiology and biotechnology , endocrinology , medicine , receptor , biochemistry , transcription factor , gene
In cells from the adrenal medulla, angiotensin II (AII) regulates both the activity and mRNA levels of catecholamine biosynthetic enzymes whose expression is thought to be under the control of cAMP‐responsive element (CRE) binding protein (CREB). In this study, we evaluated the effect of AII stimulation on CREB phosphorylation at Ser133 (pCREB) in bovine adrenal chromaffin cells (BACC). We found that AII produces a rapid and AII type‐1 receptor (AT1)‐dependent increase in pCREB levels, which is blocked by the MEK1/2 inhibitor U0126 but not by H‐89, SB203580 or KN‐93, suggesting that it is mediated by the extracellular‐regulated protein kinases 1 and 2 (ERK1/2) and not by cAMP‐dependent protein kinase (PKA), p38 mitogen‐activated protein kinase (p38MAPK) or Ca 2+ /calmodulin‐dependent protein kinases (CaMKs) dependent pathways. Gel‐shift experiments showed that the increase in pCREB levels is accompanied by an ERK1/2‐dependent upregulation of CRE‐binding activity. We also found that AII promotes a rapid and reversible increase in the activity of the non‐receptor tyrosine kinase Src and that the inhibition of this enzyme completely blocks the AII‐induced phosphorylation of ERK1/2, the CREB kinase p90 RSK and CREB. Our data support the hypothesis that in BACC, AII upregulates CREB functionality through a mechanism that requires Src‐mediated activation of ERK 1/2 and p90 RSK.