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Inhibitory effect of somatostatin on neutral amino acid transport in isolated brain microvessels
Author(s) -
Cardelli Patrizia,
Fiori Anna,
Corleto Vito D.,
Savi Maria Rosaria,
Granata Filippo,
Ceci Fabrizio,
Ferraguti Giampiero,
Potenza Rosa Luisa,
Fave Gianfranco Delle,
Jensen Robert T.,
Strom Roberto
Publication year - 2001
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2001.00412.x
Subject(s) - somatostatin , receptor , somatostatin receptor , medicine , amino acid , neuropeptide , antagonist , endocrinology , chemistry , in vivo , blood–brain barrier , inhibitory postsynaptic potential , biology , biochemistry , central nervous system , microbiology and biotechnology
In the presence of somatostatin‐14 or some of its receptorial agonists, the uptake of large neutral amino acids by isolated brain microvessels was found to be inhibited up to 50%, no other transport system being affected. Although the luminal and abluminal sides of brain endothelial cells are both capable of taking up large neutral amino acids, only uptake from the abluminal side appears to be inhibited by somatostatin. The involvement of a type‐2 somatostatin receptor was suggested by assays with a series of receptor‐specific somatostatin agonists, and was confirmed by the release of inhibition caused by a specific type‐2 receptor antagonist. A type‐2‐specific mRNA was indeed shown to be present in both bovine brain microvessels ex vivo and primary cultures of endothelial cells from rat brain microvessels.