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Gα olf is necessary for coupling D1 and A2a receptors to adenylyl cyclase in the striatum
Author(s) -
Corvol J. C.,
Studler J. M.,
Schonn J. S.,
Girault J. A.,
Hervé D.
Publication year - 2001
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2001.00201.x
Subject(s) - medicine , endocrinology , nucleus accumbens , adenylyl cyclase , dopamine , adenosine a2a receptor , dopamine receptor d1 , dopamine receptor d2 , adenosine , cgs 21680 , agonist , chemistry , biology , basal ganglia , striatum , receptor , stimulation , adenosine receptor , central nervous system
In the brain, dopamine and adenosine stimulate cyclic AMP (cAMP) production through D1 and A2a receptors, respectively. Using mutant mice deficient in the olfactory isoform of the stimulatory GTP‐binding protein α subunit, Gα olf , we demonstrate here the obligatory role of this protein in the adenylyl cyclase responses to dopamine and adenosine in the caudate putamen. Responses to dopamine were also dramatically decreased in the nucleus accumbens but remained unaffected in the prefrontal cortex. Moreover, in the caudate putamen of mice heterozygous for the mutation, the amounts of Gα olf were half of the normal levels, and the efficacy of dopamine‐ and CGS 21680 A 2 agonist‐stimulated cAMP production was decreased. Together, these results identify Gα olf as a critical parameter in the responses to dopamine and adenosine in the basal ganglia.