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Differential gene expression of β‐1,4‐galactosyltransferases I, II and V during mouse brain development
Author(s) -
Nakamura Naohito,
Yamakawa Naomi,
Sato Takeshi,
Tojo Hideaki,
Tachi Chikashi,
Furukawa Kiyoshi
Publication year - 2001
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2001.00004.x
Subject(s) - biology , microbiology and biotechnology , northern blot , gene expression , beta (programming language) , gene , in situ hybridization , western blot , glycoprotein , biochemistry , computer science , programming language
Since most brain glycoproteins from β‐1,4‐galactosyltransferase (β‐1,4‐GalT) I knockout mice were galactosylated without apparent reduction the gene expression of novel β‐1,4‐GalTs II and V which are involved in N‐linked oligosaccharide biosynthesis in addition to β‐1,4‐GalT I was studied during mouse brain development. Isolation and characterization of β‐1,4‐GalT II and V cDNAs from mouse brains indicates that they are also functioning in the brain. Northern blot analysis revealed that the β‐1,4‐GalT I gene is expressed mainly in mid‐embryonic stages, while the expression level of β‐1,4‐GalT II transcript remains constant and of β‐1,4‐GalT V transcript increases during mouse brain development after birth. In situ hybridization revealed that β‐1,4‐GalT II and V signals are present in most neural cells, with a marked difference between them in the hippocampus of adult mouse brain tissue. The differential gene expression of β‐1,4‐GalTs I, II and V during mouse brain development could affect the differential galactosylation of brain glycoproteins, as revealed by lectin blot analysis.