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C 3 ‐Fullero‐ tris ‐Methanodicarboxylic Acid Protects Cerebellar Granule Cells from Apoptosis
Author(s) -
Bisaglia M.,
Natalini B.,
Pellicciari R.,
Straface E.,
Malorni W.,
Monti C.,
Franceschi D.,
Schettini G.
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.741197.x
Subject(s) - tris , apoptosis , chemistry , cerebellum , granule (geology) , neuroscience , microbiology and biotechnology , biophysics , biochemistry , biology , paleontology
Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C 3 ‐fullero‐ tris ‐methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K + concentration from 25 to 5 m M for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration‐dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO 4 ‐induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 m M K + with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.