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Lithium Ions Up‐Regulate mRNAs Encoding Dense‐Core Vesicle Proteins in Nerve Growth Factor‐Differentiated PC12 Cells
Author(s) -
Cordeiro Mara L.,
Umbach Joy A.,
Gundersen Cameron B.
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0752622.x
Subject(s) - synaptophysin , lithium (medication) , microbiology and biotechnology , vesicular transport protein , synaptic vesicle , biology , nerve growth factor , messenger rna , vesicle , chemistry , endocrinology , biochemistry , gene , immunohistochemistry , receptor , immunology , membrane
We recently reported that lithium ions induced anup‐regulation of cysteine string protein (CSP) gene expression in nerve growthfactor (NGF)‐differentiated PC12 cells but not in undifferentiated cells.Concomitantly, expression of two other proteins of regulated secretorypathways, synaptophysin (SY) and SNAP‐25, was unaffected by lithium. To assessfurther the specificity of this effect of lithium, we used cDNA arrays. Ourdata indicate that lithium ions increase the level of mRNA for proteins suchas secretogranin II and vesicular monoamine transporter 1 that arepreferentially associated with large densecore secretory vesicles (LDCVs)without affecting mRNAs for proteins predominantly affiliated with smallsynaptic‐like vesicles, including the vesicular acetylcholine transporter andSY. This action of lithium is detected in NGF‐differentiated PC12 cells butnot in undifferentiated cells. These observations suggest that lithium ionsmodulate the turnover of LDCVs, and this may play a role in mediating thetherapeutic action of lithium in manic‐depressive illness.