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Contrasting Patterns and Cellular Specificity of Transcriptional Regulation of the Nuclear Receptor Nerve Growth Factor‐Inducible B by Haloperidol and Clozapine in the Rat Forebrain
Author(s) -
Beaudry Geneviève,
Langlois MarieClaire,
Weppe Isabelle,
Rouillard Claude,
Lévesque Daniel
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0751694.x
Subject(s) - nucleus accumbens , striatum , haloperidol , forebrain , clozapine , in situ hybridization , antipsychotic , dopamine receptor d2 , medicine , endocrinology , basal ganglia , neuroscience , sulpiride , dopaminergic , biology , chemistry , receptor , psychology , dopamine , schizophrenia (object oriented programming) , messenger rna , central nervous system , psychiatry , biochemistry , gene
This study was designed to investigate the possible involvement of members of the nuclear receptor family of transcription factors in the effects of antipsychotic drugs used in the treatment of schizophrenia. We have identified, using RT‐PCR screening, an important modulation of nerve growth factor‐inducible B (NGFI‐B) mRNA levels by typical and atypical neuroleptics in the rat forebrain. NGFI‐B, a member of the nuclear receptor family, can be observed in target structures of dopaminergic pathways. Using in situ hybridization, we also demonstrate that typical and atypical antipsychotics induced contrasting patterns of expression of NGFI‐B after both acute and chronic administration. An acute treatment with clozapine or haloperidol induces high NGFI‐B mRNA levels in the prefrontal and cingulate cortices and in the nucleus accumbens shell. However, haloperidol, but not clozapine, dramatically increases NGFI‐B expression in the dorsolateral striatum. In contrast, chronic treatment with clozapine reduces NGFI‐B expression below basal levels in the rat forebrain, whereas haloperidol still induces high NGFI‐B mRNA levels in the dorsolateral striatum. Finally, using a double in situ hybridization technique, we show that acute administration of both neuroleptics increases NGFI‐B expression in neurotensin‐containing neurons in the nucleus accumbens shell, whereas the effects of haloperidol in the dorsolateral striatum are mainly observed in enkephalin‐containing neurons. These results are the first demonstration that members of the nuclear receptor family of transcription factors could play an important role in the effects of antipsychotic drugs.