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Differential Expression of D 1A and D 1B Dopamine Receptor mRNAs in the Developing Avian Retina
Author(s) -
Soares Heline Costa,
De Melo Reis Ricardo Augusto,
De Mello Fernando Garcia,
Ventura Ana Lucia Marques,
Kurtenbach Eleonora
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0751071.x
Subject(s) - retina , synaptogenesis , biology , receptor , microbiology and biotechnology , dopamine receptor , dopaminergic , complementary dna , dopamine , medicine , endocrinology , genetics , neuroscience , gene
In the chick retina, the D1 dopaminergic system differentiates very early, as shown by receptor‐mediated increases in intracellular cyclic AMP concentration and the presence of [ 3 H]SCH23390‐specific binding sites. Here, we characterized, by RT‐PCR, the expression of defined D1 receptor subtypes D 1A , D 1B , and D 1D during the development of the chick retina. Total RNA was extracted from retinas of 6‐day‐old embryos (E6) to 1‐day‐old hatched chickens and reverse‐transcribed. The resulting cDNA was amplified using D 1A ‐, D 1B ‐, or D 1D ‐specific primers, and the PCR‐amplified products were analyzed by electrophoresis. The fragment corresponding to D 1A receptor was detected in developing retina as early as E7, whereas the fragment corresponding to D 1B was observed starting around E10. No PCR product corresponding to D 1D was observed in the retina, although it was detected in chick brain. As synaptogenesis in chick retina begins after E11 and [ 3 H]SCH 23390 D1 binding sites increase after this stage, the present results show that expression of D 1B receptor increases during synaptogenesis, whereas D 1A is the receptor subtype associated with the D1‐like actions of dopamine early in retina development.