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Identification and Cataloging of Genes Induced by Long‐Lasting Long‐Term Potentiation in Awake Rats
Author(s) -
Matsuo Ryota,
Murayama Akiko,
Saitoh Yoshito,
Sakaki Yoshiyuki,
Inokuchi Kaoru
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0742239.x
Subject(s) - long term potentiation , biology , ltp induction , gene , gene expression , complementary dna , genetics , microbiology and biotechnology , receptor
Maintenance of long‐term potentiation (LTP) requires de novo gene expression. Here we report the direct isolation, using PCR‐differential display, of genes whose expression level was altered after induction of long‐lasting LTP in the hippocampus of freely moving awake rats. Differential display using 480 primer combinations revealed 17 cDNA bands that showed a reproducible change in expression level. These cDNAs represented at least 10 different genes (termed RM1‐10 ), all of which showed up‐regulation at 75 min after LTP induction and a return to basal expression levels within 24 h. Three of these genes were known only from expressed sequence tags ( RM1‐3 ), two were known genes whose up‐regulation by LTP has not been described ( GADD153/CHOP and ler5 ), and five were known genes whose up‐regulation by LTP has already been reported ( MAPK phosphatase, NGFI‐A/zif268, vesl‐1S/homer‐1a, Ag2 , and krox‐20 ). We characterized the expression profiles of genes in the two former categories with respect to NMDA receptor dependency, tissue specificity, and developmental regulation using northern blotting and semiquantitative RT‐PCR. The up‐regulation of all five of these genes was NMDA receptor‐dependent and correlated with the persistence of LTP, suggesting that these genes may play functional roles in prolonged LTP maintenance.