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Identification of Variants and Dual Promoters of Murine Serine/Threonine Kinase KKIAMRE
Author(s) -
Sassa Takayuki,
Gomi Hiroshi,
Sun William,
Ikeda Toshio,
Thompson Richard F.,
Itohara Shigeyoshi
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0741809.x
Subject(s) - promoter , exon , biology , alternative splicing , gene isoform , gene , complementary dna , microbiology and biotechnology , gene expression , threonine , genetics , serine , phosphorylation
KKIAMRE is a serine/threonine protein kinase whose transcripts increase in the deep cerebellar nuclei of the rabbit after eyeblink conditioning, a model of associative learning and memory. We here characterized the expression, isoforms, and promoters of murine KKIAMRE gene. The expression of KKIAMRE was detected, by in situ hybridization and immunohistochemistry, in neurons in various brain regions including deep cerebellar nuclei. The gene spans ∼40 kb and consists of 15 exons. Analysis of cDNA clones revealed multiple variants, having diversity in the putative carboxy‐terminal regulatory domain, generated by alternative splicing and intraexonal termination. Furthermore, they had alternative 5′ noncoding sequences. Primer extension, RNase protection, and transient expression assays revealed that two alternative promoters linked to distinct noncoding exons direct the expression of KKIAMRE. The gene was mapped on chromosomes 5 and 4 in mouse and human, respectively.