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Brief Exposure to Neurotrophins Produces a Calcium‐Dependent Increase in Choline Acetyltransferase Activity in Cultured Rat Septal Neurons
Author(s) -
ner Doris,
Barrett Ellen F.,
Barrett John N.
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0740988.x
Subject(s) - choline acetyltransferase , neurotrophin , nerve growth factor , neurotrophic factors , medicine , endocrinology , stimulation , brain derived neurotrophic factor , cholinergic , extracellular , premovement neuronal activity , biology , bapta , cholinergic neuron , chemistry , neuroscience , microbiology and biotechnology , receptor
We demonstrate that brief (30‐min) exposure of cultured embryonic rat septal neurons to neurotrophins (NTs) increases choline acetyltransferase (ChAT) activity by 20‐50% for all tested NTs (nerve growth factor, brain‐derived neurotrophic factor, neurotrophin‐3, and neurotrophin‐4, each at 100 ng/ml). The increase in ChAT activity was first detected 12 h after NT exposure, persisted at least 48 h, and was not mediated by increased neuronal survival or action‐potential activity. Under some conditions, the response to brief NT exposure was as great as that produced by continuous exposure. NT stimulation of ChAT activity was inhibited by inhibitors of p75‐ and Trk kinase‐mediated signaling, by removal of extracellular Ca 2+ during the period of NT exposure, and by buffering intracellular Ca 2+ with BAPTA. Application of nerve growth factor and brain‐derived neurotrophic factor transiently increased [Ca 2+ ] within a subpopulation of neurons. NT stimulation of ChAT activity was not affected significantly by cyclic AMP agonists or antagonists. These findings suggest that brief exposure to NTs can have a long‐lasting effect on cholinergic transmission, and that this effect requires Ca 2+ , but not cyclic AMP.