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A Proteolipid Protein‐Specific Pre‐mRNA ( Ppm‐1 ) Contains Intron 3 and Is Up‐Regulated During Myelination in the CNS
Author(s) -
Vouyiouklis Demetrius A.,
Barrie Jennifer A.,
Griffiths Ian R.,
Thomson Christine E.
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0740940.x
Subject(s) - messenger rna , biology , proteolipid protein 1 , rna splicing , precursor mrna , intron , gene expression , microbiology and biotechnology , gene isoform , primary transcript , gene , gene product , alternative splicing , oligodendrocyte , rna , myelin basic protein , genetics , myelin , endocrinology , central nervous system
Alternative splicing of the precursor for messenger RNA (pre‐mRNA) is a common process utilised by higher eukaryotes to modulate gene expression. A single primary transcript may generate several proteins with distinct functions, expressed in tissue‐specific, developmental patterns. This article describes an oligodendrocyte‐specific pre‐mRNA product of proteolipid protein gene ( Plp ) transcription, which is the precursor for Plp but not Dm20 mRNA in the CNS. This Plp ‐specific pre‐mRNA ( Ppm‐1 ) includes the intact intron 3 of the Plp gene. It is first expressed during active myelination, and it localises to the nucleus of oligodendrocytes, in both normal and jimpy ( jp ) murine CNS. In addition to mouse, Ppm‐1 is found also in rat and dog, but not toad or trout. Our work suggests that alternative splicing of the Plp gene primary transcript follows a branching pattern, resulting in the presence of at least one Plp isoform‐specific pre‐mRNA molecule, Ppm‐1 . Therefore, Dm20 mRNA may be the product of a divergent set of pre‐mRNA splicing events.